Leptin Protein (LEP)
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- 抗原 See all Leptin (LEP) 蛋白
- Leptin (LEP)
- 蛋白类型
- Recombinant
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 012-001-320-0200
- Supplier
- Rockland
- 原理
- Rat Leptin Recombinant Protein
- 纯化方法
- Leptin purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- 纯度
- 95,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is currently undetermined.
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- Discover our top product LEP 蛋白
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Leptin (LEP) (AA 22-167) protein (Fc Tag)
LEP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leptin (LEP) proteinLEP 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) protein (His tag)LEP 宿主: Goldfish 宿主: 酵母菌 Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (His tag)LEP 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 428-635) protein (His tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leptin (LEP) (AA 22-167) protein (GST tag)LEP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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- 应用备注
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Other: User Optimized
Application_Note: Leptin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Leptin in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
- 浓度
- 0.1 mg/mL
- 缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- Leptin (LEP)
- 别名
- Lep (LEP 产品)
- 背景
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Synonyms: Obesity protein
Background: Leptin is a protein that is thought to have a critical role in the physiologic regulation of body weight via its capacity to inhibit food intake and stimulate energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant rat Leptin is a non-glycosylated protein, containing 147 amino acids, with a molecular weight of 16.2 kDa.
- UniProt
- P50596
- 途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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