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KIT Ligand Protein (KITLG)

Rockland 010-001-w20-0100 KITLG 宿主: 小鼠 宿主: 大肠杆菌(E. Coli) Recombinant 98,00%
Rockland
产品编号 ABIN6700197
Supplier Product No.: 010-001-w20-0100
发货至: 中国
  • 抗原 See all KIT Ligand (KITLG) 蛋白
    KIT Ligand (KITLG)
    蛋白类型
    Recombinant
    宿主
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    小鼠
    资源
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    大肠杆菌(E. Coli)
    Supplier Product No.
    010-001-w20-0100
    Supplier
    Rockland
    原理
    Mouse Stem Cell Factor Recombinant Protein
    纯化方法
    Stem Cell Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    纯度
    98,00%
    内毒素水平
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by the dose-dependent proliferation of human TF-1 cells and is typically less than 10 ng/mL.
    Top Product
    Discover our top product KITLG 蛋白
  • 应用备注

    Other: User Optimized

    Application_Note: Stem Cell Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Stem Cell Factor in immunological assays.

    说明

    Suggested_Applications: Cellular Assay, IHC, WB

    Other_Performance_Data:

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 100 μL

    浓度
    0.1 mg/mL
    缓冲液
    Lyophilized with 20 mM sodium phosphate, pH 6.5.
    储存液
    Without preservative
    储存条件
    -20 °C
    储存方法
    Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    有效期
    6 months
  • Turnis, Kaminska, Smith, Kartchner, Vogel, Laxton, Ashmun, Ney, Opferman: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).

  • 抗原
    KIT Ligand (KITLG)
    别名
    Kitlg (KITLG 产品)
    背景

    Synonyms: c-Kit Ligand, Hematopoietic growth factor KL, KL, Steel Factor, Stem cell factor (SCF), Mast cell growth factor(MGF)

    Background: Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant mouse SCF is a non-glycosylated protein, containing 164 amino acids, with a molecular weight of 18.3 kDa.

    UniProt
    P20826
    途径
    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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