C-JUN Protein (GST tag)
-
- 抗原 See all C-JUN (JUN) 蛋白
- C-JUN (JUN) (Jun Proto-Oncogene (JUN))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This C-JUN protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r71s
- Supplier
- Rockland
- 原理
- c-JUN recombinant protein-GST fusion protein
- 纯化方法
- Recombinant human c-JUN (1-79) expressed in E. coli cells using an N-Terminal GST Protein. The purity was determined to be >50% by densitometry.
- 纯度
- >50%
- Top Product
- Discover our top product JUN 蛋白
-
Jun Proto-Oncogene (JUN) (AA 1-241) protein (His tag)
JUN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Jun Proto-Oncogene (JUN) (AA 75-274) protein (His tag)JUN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Jun Proto-Oncogene (JUN) (AA 1-331) protein (GST tag)JUN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
-
- 应用备注
-
Western_Blot_Dilution: User Optimized
Other: Kinase Assay-User Optimized
Application_Note: c-JUN Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 35 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- c-JUN Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
-
- 抗原
- C-JUN (JUN) (Jun Proto-Oncogene (JUN))
- 别名
- JUN (JUN 产品)
- 背景
-
Synonyms: AP1, Transcription factor AP-1, Activator protein 1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39
Background: c-Jun is a proto-oncogene that forms a complex with c-Fos which regulates transcription from promoters containing AP-1 activation elements (1). c-Jun has specific DNA binding activity and following in vitro translation, c-Jun binds as a homodimer to the AP-1 DNA site. The transactivating function of c-Jun is acutely regulated by a wide variety of cellular signals via modulation of phosphorylation of two serines (63 and 73). Jun N-terminal domain kinases (JNKs), are responsible for mediating S63/73 phosphorylation on c-Jun in response to a variety of cellular stimuli including TNF-a, heat stress and u.v. light (2). c-JUN Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, JNK/SAPK Pathway, Neurobiology, and NfkB Pathway research.
- NCBI登录号
- NM_002228
- 途径
- MAPK Pathway, RTK signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins
-
