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IL-27 蛋白

Rockland 010-f01-b54-0100 Animal-Free IL27 宿主: 小鼠 宿主: 大肠杆菌(E. Coli) Recombinant 97,00% SDS
Rockland
产品编号 ABIN6700106
Supplier Product No.: 010-f01-b54-0100
发货至: 中国
  • 抗原 See all IL-27 (IL27) 蛋白
    IL-27 (IL27) (Interleukin 27 (IL27))
    蛋白类型
    Recombinant
    宿主
    • 7
    • 6
    • 1
    小鼠
    资源
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    大肠杆菌(E. Coli)
    应用范围
    SDS-PAGE (SDS)
    Supplier Product No.
    010-f01-b54-0100
    Supplier
    Rockland
    原理
    Mouse Interleukin-27 (p28) Recombinant Protein (Animal Free)
    纯化方法
    The p28 subunit of IL-27 is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 97% as determined by reducing and non-reducing SDS-PAGE.
    纯度
    97,00%
    内毒素水平
    Measured by LAL is typically ≤ 1 EU/μg protein (with a 50% confidence range).
    质量等级
    Animal-Free
    Biological Activity Comment
    Mouse p28 biological activity was measured via dose-dependent inhibition of TGFß and IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50ng/ml of Mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay. no acceptance criteria limits established.
    Top Product
    Discover our top product IL27 蛋白
  • 应用备注

    Other: User Optimized

    Application_Note: Interleukin-27 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-27 in immunological assays.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 100 μL

    缓冲液
    Buffer Formulation: 10 mM sodium bicarbonate, pH 8.5.
    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    有效期
    6 months
  • 抗原
    IL-27 (IL27) (Interleukin 27 (IL27))
    别名
    Il27 (IL27 产品)
    背景

    Synonyms: IL-30, P28, IL-27 p28 subunit

    Background: The p28 subunit of IL-27 (IL-27/p28), also known as Interleukin-30 (IL-30), is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), p28 forms a heterodimer known as IL-27. p28 is thought to be a pro-inflammatory cytokine inducing immuno-modulatory effects, but the details of these effects are currently unclear and research is focused on delineating its specific biological functions. Recombinant mouse p28 is a non-glycosylated protein, containing 207 amino acids, with a molecular weight of 23.7 kDa.

    UniProt
    Q8K3I6
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