Interleukin 17a 蛋白
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- 抗原 See all Interleukin 17a (IL17A) 蛋白
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-b32
- Supplier
- Rockland
- 原理
- IL-17A Human Recombinant Protein
- 纯化方法
- Recombinant protein corresponds to amino acids 19 - 155 of mature human IL-17A. Its molecular weight is 15,667 Daltons. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
- 纯度
- 90,00%
- 内毒素水平
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
- Biological Activity Comment
- Human IL-17A is fully biologically active when compared to standards via dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts. The ED50 for recombinant human IL-17A in this assay was 1.4-2.1 ng/mL.
- Top Product
- Discover our top product IL17A 蛋白
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Interleukin 17A (IL17A) (AA 24-155) protein (His tag)
IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17A (IL17A) (AA 24-155) protein (His-Avi Tag,Biotin)IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (AA 24-155) protein (His-Avi Tag)IL17A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 92 % as determined by HPLC
Interleukin 17A (IL17A) (AA 26-158) protein (His-Avi Tag,Biotin)IL17A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (Active) protein (His tag)IL17A 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 17A (IL17A) (Active) protein (His tag)IL17A 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 26-158) protein (His-Avi Tag)IL17A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His tag)IL17A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His tag,AVI tag,Biotin)IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 26-158) protein (His tag)IL17A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- 应用备注
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Other: User Optimized
Application_Note: IL-17A protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17A in immunological assays.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 250 μL
- 浓度
- 0.1 mg/mL
- 缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- 别名
- IL17A (IL17A 产品)
- 背景
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Synonyms: Interleukin-17 cytokine, IL-17, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8.
Background: Interleukin17-A is a proinflammatory cytokine produced by activated T cells and exists as a homodimer. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL-17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants: IL17RA, IL17RB, and IL17RC.
- UniProt
- Q16552
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