IL12 蛋白
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- 抗原 See all IL12 蛋白
- IL12 (Interleukin 12 (IL12))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-v51-0010
- Supplier
- Rockland
- 原理
- Mouse Interleukin-12 Recombinant Protein
- 纯化方法
- Interleukin-12 purity was determined to be greater than 95% as determined by reducing and non-reducing SDS-pAGE.
- 纯度
- 95,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the induced expression of CD25 on CD8+ mouse T cells and is typically detectable starting at 0.1 ng/mL.
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- Discover our top product IL12 蛋白
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- 应用备注
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Other: User Optimized
Application_Note: Interleukin-12 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-12 in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
- Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- IL12 (Interleukin 12 (IL12))
- 别名
- Il12a/Il12b (IL12 产品)
- 背景
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Synonyms: NKSF, CLMF
Background: Interleukin-12 (IL-12) is an important mediator of cellular-immunity produced by dendritic cells, macrophages and B cells when stimulated with antigen. IL-12 is involved in differentiating precursor helper T cells to Th1 and/or Th2 subtypes and promotes the production of IFN gamma and TNF alpha. Mouse IL-12 is active on human and mouse cells. Recombinant mouse IL-12 is a glycosylated, disulfide-linked heterodimer. It is containing one p35 su
- UniProt
- P43432
- 途径
- JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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