IL-1 beta 蛋白
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- 抗原 See all IL-1 beta (IL1B) 蛋白
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- 蛋白类型
- Recombinant
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 012-001-301-0010
- Supplier
- Rockland
- 原理
- Rat Interleukin-1 beta Recombinant Protein
- 纯化方法
- Interleukin-1 beta purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- 纯度
- 97,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse D10S cells and is typically less than 1 ng/mL.
- Top Product
- Discover our top product IL1B 蛋白
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Interleukin 1, beta (IL1B) (AA 117-269) protein (His-Avi Tag)
IL1B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Interleukin 1, beta (IL1B) (AA 117-269) protein (His-Avi Tag,Biotin)IL1B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 1, beta (IL1B) (AA 1-269) protein (GST tag)IL1B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 1, beta (IL1B) (AA 117-269) protein (Fc Tag)IL1B 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 1, beta (IL1B) (AA 113-271) protein (His tag)IL1B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Interleukin 1, beta (IL1B) (AA 115-267) protein (His tag)IL1B 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 1, beta (IL1B) (AA 116-268) protein (His tag)IL1B 宿主: 马 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 1, beta (IL1B) (AA 114-266) protein (His tag)IL1B 宿主: 山羊 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 1, beta (IL1B) (AA 115-266) protein (His tag)IL1B 宿主: 犬 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 1, beta (IL1B) (AA 106-267) protein (His tag)IL1B 宿主: 小鸡 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- 应用备注
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Other: User Optimized
Application_Note: Interleukin-1-beta Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-1-beta in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- 别名
- Il1b (IL1B 产品)
- 背景
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Synonyms: Catabolin, LAF, EP, LEM, MCF
Background: Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine, produced in response to inflammatory agents by a variety of cells, including, monocytes, macrophages, and dendritic cells (DCs). IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant rat IL-1β is a non-glycosylated protein, containing 152 amino acids, with a molecular weight of 17.3 kDa.
- UniProt
- Q63264
- 途径
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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