IL1A 蛋白
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- 抗原 See all IL1A 蛋白
- IL1A (Interleukin 1 alpha (IL1A))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-v24-0010
- Supplier
- Rockland
- 原理
- Human Interleukin-1-alpha Recombinant Protein
- 纯化方法
- Interleukin-1-alpha purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- 纯度
- 97,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the stimulation of D10S.G4.1 cells and is typically 1-6 pg/mL.
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- Discover our top product IL1A 蛋白
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- 应用备注
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Other: User Optimized
Application_Note: Interleukin-1-alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-1-alpha in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- IL1A (Interleukin 1 alpha (IL1A))
- 别名
- IL1A (IL1A 产品)
- 背景
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Synonyms: IL-1F1, FAF, BAF, LEM, LAF, Hematopoietin-1
Background: Interleukin-1 alpha (IL-1α) is constitutively expressed by epithelial cells, but can also be produced by most other cells upon stimulation. IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant human IL-1α is a non-glycosylated single chain protein, containing 159 amino acids, with a molecular weight of 18 kDa.
- UniProt
- P01583
- 途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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