Interferon gamma Protein (IFNG)
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- 抗原 See all Interferon gamma (IFNG) 蛋白
- Interferon gamma (IFNG)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- Supplier Product No.
- 009-001-v20-0020
- Supplier
- Rockland
- 原理
- Human Interferon-gamma Recombinant Protein
- 纯化方法
- Interferon-gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- 纯度
- 95,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is measured in antiviral assays using virally infected L929 cells and is typically less than 0.1 ng/mL.
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- Discover our top product IFNG 蛋白
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Interferon gamma (IFNG) protein (His-Avi Tag)
IFNG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interferon gamma (IFNG) protein (His-Avi Tag,Biotin)IFNG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Interferon gamma (IFNG) (Active) proteinIFNG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) proteinIFNG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interferon gamma (IFNG) (AA 24-166) protein (His tag)IFNG 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (AA 1-144) protein (His tag)IFNG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG 宿主: Hamster 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (Active) protein (Fc Tag)IFNG 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 92 % as determined by SDS-PAGE Active
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- 应用备注
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Other: User Optimized
Application_Note: Interferon-gamma Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
- 浓度
- 0.1 mg/mL
- 缓冲液
- Lyophilized from an aqueous solution containing 10 mM Sodium Phosphate, 50 mM Sodium Chloride, pH 7.5.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- Interferon gamma (IFNG)
- 别名
- IFNG (IFNG 产品)
- 背景
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Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF
Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFN-γ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Human IFNγ does not show cross-reactivity with mouse. Recombinant human IFNγ is a non-glycosylated protein, containing 144 amino acids, with a molecular weight of 16.88 kDa.
- UniProt
- P01579
- 途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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