GDF5 蛋白
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- 抗原 See all GDF5 蛋白
- GDF5 (Growth Differentiation Factor 5 (GDF5))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-u94-0010
- Supplier
- Rockland
- 原理
- Human Growth and Differentiation Factor-5 Recombinant Protein
- 纯化方法
- Growth and Differentiation Factor-5 purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- 纯度
- 98,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the ability to induce alkaline phosphatase activity in ATDC5 cells is typically 0.1-1 μg/mL.
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- Discover our top product GDF5 蛋白
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- 应用备注
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Other: User Optimized
Application_Note: Growth and Differentiation Factor-5 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Growth and Differentiation Factor-5 in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- GDF5 (Growth Differentiation Factor 5 (GDF5))
- 别名
- GDF5 (GDF5 产品)
- 背景
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Synonyms: Cartilage-derived morphogenetic protein 1
Background: Growth/Differentiation Factor 5 (GDF-5) is a growth factor that regulates cell proliferation and differentiation in embryonic and adult tissues. GDF-5 is part of the TGF family of proteins and is closely related to the BMP family of proteins. Recombinant human GDF-5 is a non-glycosylated homodimer, containing two 120 amino acids chains, with a total molecular weight of 27.4 kDa. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys.
- UniProt
- P43026
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