Follistatin Protein (FST)
-
- 抗原 See all Follistatin (FST) 蛋白
- Follistatin (FST)
- 蛋白类型
- Recombinant
-
宿主
- 小鼠
-
资源
- 大肠杆菌(E. Coli)
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-u88-0005
- Supplier
- Rockland
- 原理
- Mouse Follistatin Recombinant Protein
- 纯化方法
- Follistatin purity was determined to be greater than 90% as determined by analysis by reducing and non-reducing SDS-pAGE.
- 纯度
- 90,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.
- Top Product
- Discover our top product FST 蛋白
-
-
- 应用备注
-
Other: User Optimized
Application_Note: Follistatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Follistatin in immunological assays.
- 说明
-
Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
- 缓冲液
- Buffer Formulation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
-
- 抗原
- Follistatin (FST)
- 别名
- Fst (FST 产品)
- 背景
-
Synonyms: FS, activin-binding protein, FSH-suppressing protein (FSP)
Background: Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors. Recombinant mouse follistatin is a non-glycosylated protein, containing 289 amino acids, with a molecular weight of 31.6 kDa.
- UniProt
- P47931
- 途径
- Negative Regulation of Hormone Secretion
-