Cyclin O Protein (CCNO) (GST tag)
-
- 抗原 See all Cyclin O (CCNO) 蛋白
- Cyclin O (CCNO)
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- Insect cells (Sf9)
- 标记
- This Cyclin O protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r82s
- Supplier
- Rockland
- 原理
- CyclinO recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human CyclinO was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
- 纯度
- >95%
- Top Product
- Discover our top product CCNO 蛋白
-
-
- 应用备注
-
Western_Blot_Dilution: User Optimized
Application_Note: CyclinO Protein is suitable for use in Western Blot. Expect a band approximately ~ 69 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.1 μg/μL
- 缓冲液
- CyclinO Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
-
- 抗原
- Cyclin O (CCNO)
- 别名
- CCNO (CCNO 产品)
- 背景
-
Synonyms: CCNO, CCNU, UDG2, Cyclin-O
Background: Cyclin O belongs to the highly conserved cyclin family. These family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclin O plays an important role in the processes of transcription. Cyclin O also plays an important role in DNA repair and cell cycle progression. Cyclin O localizes exclusively in the nucleus (1). Cyclin O shows significant uracil-DNA glycosylase activity in which the activity of in vitro translated UDG2 was not as robust as that of the mitochondrial isoform of UNG (2). Cyclin O Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
- NCBI登录号
- NM_021147
-
