Cyclin K Protein (CCNK) (GST tag)
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- 抗原 See all Cyclin K (CCNK) 蛋白
- Cyclin K (CCNK)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This Cyclin K protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r81s
- Supplier
- Rockland
- 原理
- CyclinK recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human CyclinK was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
- 纯度
- >85%
- Top Product
- Discover our top product CCNK 蛋白
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Cyclin K (CCNK) (AA 1-354) protein (GST tag)
CCNK 宿主: 人 宿主: 小麦胚 Recombinant WB, AA, AP, ELISA
Cyclin K (CCNK) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CCNK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: CyclinK Protein is suitable for use in Western Blot. Expect a band approximately ~ 67 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1 μg/μL
- 缓冲液
- CyclinK Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- Cyclin K (CCNK)
- 别名
- CCNK (CCNK 产品)
- 背景
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Synonyms: CCNK, CPR4, MGC9113, Cyclin-K
Background: CyclinK is a member of the cyclin family which can regulate transcription through their association with and activation of cyclin-dependent kinases (CDK). The CDK/CyclinK complex can phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II (1). CyclinK is associated with potent CTD kinase and CDK kinase activities in vitro and coimmunoprecipitates with POLR2A. CyclinK is a new member of the transcription cyclin family which may play a dual role in regulating CDK and RNA polymerase II activities (2). Cyclin K Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
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