Cyclin H Protein (CCNH) (GST tag)
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- 抗原 See all Cyclin H (CCNH) 蛋白
- Cyclin H (CCNH)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This Cyclin H protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r80s
- Supplier
- Rockland
- 原理
- CyclinH recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full length human CyclinH was expressed in insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
- 纯度
- >90%
- Top Product
- Discover our top product CCNH 蛋白
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: CyclinH Protein is suitable for use in Western Blot. Expect a band approximately ~ 39 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1 μg/μL
- 缓冲液
- CyclinH Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- Cyclin H (CCNH)
- 别名
- CCNH (CCNH 产品)
- 背景
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Synonyms: CCNH, CAK, p34, p37, Cyclin-H, MO15-associated protein
Background: Cyclin H is related to the highly conserved cyclin family. Cyclin family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Cyclin H forms a complex with CDK7 kinase and the ring finger protein MAT1 which is able to phosphorylate CDK2 and CDC2 kinases and thus act as a CDK-activating kinase (CAK) (1). Cdk-activating kinase complex is a component of human transcription factor TFIIH and plays an important role in the processes of transcription, DNA repair, and cell cycle progression (2). Cyclin H Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
- NCBI登录号
- NM_001239
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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