Cyclin E1 Protein (CCNE1) (GST tag)
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- 抗原 See all Cyclin E1 (CCNE1) 蛋白
- Cyclin E1 (CCNE1)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This Cyclin E1 protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r79s
- Supplier
- Rockland
- 原理
- CyclinE1 recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human CyclinE1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
- 纯度
- >90%
- Top Product
- Discover our top product CCNE1 蛋白
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Cyclin E1 (CCNE1) (AA 1-410) protein (GST tag)
CCNE1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Cyclin E1 (CCNE1) (Active) protein (GST tag,His tag)CCNE1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE Active
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- 应用备注
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Western_Blot_Dilution: User Optimized
Other: Kinase Assay-User Optimized
Application_Note: CyclinE1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 73 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- CyclinE1 is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- Cyclin E1 (CCNE1)
- 别名
- CCNE1 (CCNE1 产品)
- 背景
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Synonyms: CCNE1, CCNE, G1/S-specific cyclin-E1
Background: Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle that functions as regulators of CDK kinases. Cyclin E1 activity is required for centrosome duplication during S phase and this mechanism could coordinate centrosome reproduction with cycles of DNA synthesis and mitosis (1). The downregulation of cyclin E-CDK2 kinase activity following the G1/S-phase transition that is necessary for the maintenance of karyotypic stability. Cyclin E also has a modular centrosomal-targeting domain which is essential for promoting S phase entry in a Cdk2-independent manner (2). Cyclin E1 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
- NCBI登录号
- NM_001238
- 途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
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