Cyclin D1 Protein (CCND1) (GST tag)
(1 reference)
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- 抗原 See all Cyclin D1 (CCND1) 蛋白
- Cyclin D1 (CCND1)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Cyclin D1 protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-153s
- Supplier
- Rockland
- 原理
- CyclinD1 recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human CyclinD1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
- 纯度
- >85%
- Top Product
- Discover our top product CCND1 蛋白
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Cyclin D1 (CCND1) (AA 1-295) protein (GST tag)
CCND1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: CyclinD1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 61 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 说明
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Biochemical Assay, Functional Assay
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- CyclinD1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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: "Ribosomal protein RPL22/eL22 regulates the cell cycle by acting as an inhibitor of the CDK4-cyclin D complex." in: Cell cycle (Georgetown, Tex.), Vol. 18, Issue 6-7, pp. 759-770, (2020) (PubMed).
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: "Ribosomal protein RPL22/eL22 regulates the cell cycle by acting as an inhibitor of the CDK4-cyclin D complex." in: Cell cycle (Georgetown, Tex.), Vol. 18, Issue 6-7, pp. 759-770, (2020) (PubMed).
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- 抗原
- Cyclin D1 (CCND1)
- 别名
- CCND1 (CCND1 产品)
- 背景
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Synonyms: BCL1, PRAD1, U21B31, D11S287E, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene
Background: CyclinD1 is a member of the cyclin family of proteins that are important regulators cyclin-dependent protein kinases (CDKs) (1). CyclinD1 forms a complex and functions as a regulatory subunit of CDK4 or CDK6 which are important during cell cycle G1/S transition. CyclinD1 has been shown to interact with the tumor suppressor protein Rb and the expression of CyclinD1 is positively regulated by Rb. Mutations, amplification and overexpression of CyclinD1 have been frequently observed in a variety of tumors and suggesting a role of this protein in tumorigenesis (2). CyclinD1 is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
- NCBI登录号
- NM_053056
- 途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
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