Cyclin C Protein (CCNC) (GST tag)
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- 抗原 See all Cyclin C (CCNC) 蛋白
- Cyclin C (CCNC)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This Cyclin C protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r77s
- Supplier
- Rockland
- 原理
- CyclinC recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human CyclinC was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.
- 纯度
- >70%
- Top Product
- Discover our top product CCNC 蛋白
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Cyclin C (CCNC) (AA 1-283) protein (GST tag)
CCNC 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: CyclinC Protein is suitable for use in Western Blot. Expect a band approximately ~ 55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.05 μg/μL
- 缓冲液
- CyclinC Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- Cyclin C (CCNC)
- 别名
- CCNC (CCNC 产品)
- 背景
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Synonyms: CCNC, CycC, Cyclin-C, SRB11 homolog, hSRB11
Background: Cyclin C is a member of the cyclin family of proteins and interacts with the cyclin-dependent kinase 8 (CDK8) and induces the phosphorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The mRNA levels of Cyclin C fluctuate during the cell cycle and peak at the G1 phase. Cyclin C acts as a critical regulator of the G0/G1 transition of human Hematopoietic stem cells (1). Cyclin C can also combine with CDK3 to stimulate Rb phosphorylation at S807/811 during the G0/G1 transition, and this phosphorylation is required for cells to exit G0 efficiently (2). Cyclin C Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
- NCBI登录号
- NM_005190
- 途径
- Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha
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