G-CSF 蛋白
-
- 抗原 See all G-CSF (CSF3) 蛋白
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- 蛋白类型
- Recombinant
-
宿主
- 小鼠
-
资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-u93-0010
- Supplier
- Rockland
- 原理
- Mouse Granulocyte Colony Stimulating Factor Recombinant Protein
- 纯化方法
- Granulocyte Colony Stimulating Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- 纯度
- 98,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse M-NFS-60 cells and is typically less than 10-60 ng/mL.
- Top Product
- Discover our top product CSF3 蛋白
-
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) protein
CSF3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) protein (His tag)CSF3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 22-214) protein (His tag)CSF3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) protein (GST tag)CSF3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-208) (Active) proteinCSF3 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
-
- 应用备注
-
Other: User Optimized
Application_Note: Granulocyte Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Colony Stimulating Factor in immunological assays.
- 说明
-
Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
-
Buffer: 0.01 M Sodium Citrate, pH 3.0
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
-
- 抗原
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- 别名
- Csf3 (CSF3 产品)
- 背景
-
Synonyms: CSF-3, MGI-1G, GM-CSFβ, pluripoietin
Background: Granulocyte Colony-Stimulating Factor, or G-CSF, is a growth factor that is considered the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF, while in epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by IL-17. Human and mouse G-CSF are cross-reactive. Recombinant mouse G-CSF is a non-glycosylated protein, containing 179 amino acids, with a molecular weight of 19 kDa.
- UniProt
- P09920
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
-
