CCL2 蛋白
-
- 抗原 See all CCL2 蛋白
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- 蛋白类型
- Recombinant
-
宿主
- 大鼠
-
资源
- 大肠杆菌(E. Coli)
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 012-001-v74-0010
- Supplier
- Rockland
- 原理
- Rat Monocyte Chemotactic Protein-1 (CCL2) Recombinant Protein
- 纯化方法
- Monocyte Chemotactic protein-1 (CCL2) purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- 纯度
- 98,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the ability to chemoattract human monocytes or THP-1 cells at 4 - 15 ng/mL.
- Top Product
- Discover our top product CCL2 蛋白
-
-
- 应用备注
-
Other: User Optimized
Application_Note: Monocyte Chemotactic Protein-1 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Monocyte Chemotactic Protein-1 in immunological assays.
- 说明
-
Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
-
Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
-
- 抗原
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- 别名
- Ccl2 (CCL2 产品)
- 背景
-
Synonyms: Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, MCAF, Monocyte chemotactic protein 1 (MCP-1), Small-inducible cytokine A2
Background: Monocyte Chemotactic Protein 1 (MCP-1), also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. Recombinant rat MCP-1is a non-glycosylated protein, containing 125 amino acids, with a molecular weight of 14.1 kDa.
- UniProt
- P14844
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
-