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c-FOS Protein (GST tag)

Rockland 009-001-r95s c-Fos 宿主: 人 宿主: Insect cells (Sf9) Recombinant >90% WB
Rockland
产品编号 ABIN6699563
Supplier Product No.: 009-001-r95s
发货至: 中国
  • 抗原 See all c-FOS (c-Fos) 蛋白
    c-FOS (c-Fos) (c-Fos)
    蛋白类型
    Recombinant
    宿主
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    资源
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    Insect cells (Sf9)
    标记
    This c-FOS protein is labelled with GST tag.
    应用范围
    Western Blotting (WB)
    Supplier Product No.
    009-001-r95s
    Supplier
    Rockland
    原理
    FOS recombinant protein-GST fusion protein
    纯化方法
    Recombinant full-length human FOS was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
    纯度
    >90%
    Top Product
    Discover our top product c-Fos 蛋白
  • 应用备注

    Western_Blot_Dilution: User Optimized

    Other: Kinase Assay-User Optimized

    Application_Note: FOS Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~78 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 μg/μL
    缓冲液
    FOS Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    储存条件
    -80 °C
    储存方法
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    有效期
    12 months
  • 抗原
    c-FOS (c-Fos) (c-Fos)
    别名
    FOS (c-Fos 产品)
    背景

    Synonyms: AP-1, c-FOS, Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

    Background: FOS is a member of the FOS gene family that consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. FOS gene encodes a leucine zipper protein that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation (1). In some cases, expression of the FOS gene has also been associated with apoptotic cell death. FOS overexpression leads to decreased phosphorylation and dimerization of STAT1, which in turn downregulates p21 gene expression (2). This regulatory pathway may enhance the proliferation of lymphocytes in rheumatoid arthritis patients. FOS Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, and Neurobiology research.

    NCBI登录号
    NM_005252
    途径
    S100 Proteins
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