Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thereby regulating the contractile activity. MYLPF is critically important for fast and slow skeletal muscle development. Recombinant human MYLPF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Myosin regulatory light chain 2, skeletal muscle isoform, MRLC2, MYL11, Fast skeletal myosin light chain 2, MLC2B. NCBI no.: NP_037424