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- 抗原
- DnaK
- 蛋白类型
- Recombinant
- 产品特性
- AA 385-546
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宿主
- E. coli
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 产品特性
- Dnak(385-546), E.coli, Recombinant, E.coli
- 纯度
- > 95 % by SDS - PAGE
-
DnaK (AA 1-638), (full length) protein
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- Liquid. 25 mM Tris-HCl, pH7.5, 2 mM beta-mercaptoethanol, 1 mM EDTA.
- 储存条件
- 4 °C
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- 抗原
- DnaK
- 别名
- Dnak (385-546)
- 背景
- DNAK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus. Synonyms: Chaperone protein dnaK, HSP70, groP, grpF, seg, Heat shock protein 70, Chaperone Hsp70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,. NCBI no.: NP_414555
- 分子量
- 17.7kDa (163aa)
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