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- 抗原
- DnaK
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-384
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宿主
- E. coli
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 产品特性
- Dnak(N-term, 1-384), E.coli, Recombinant, E.coli)
- 纯度
- > 95 % by SDS - PAGE
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DnaK (AA 1-638), (full length) protein
宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 385-546) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 508-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 385-638) protein宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
DnaK (AA 1-638), (full length) protein宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
DnaK (AA 1-384), (N-Term) protein宿主: 所有种类 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5 mM DTT, 10%Glycerol.
- 储存条件
- 4 °C
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- 抗原
- DnaK
- 背景
- DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK(amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain(residues385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. The ATPase domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Synonyms: dnaK, Dnak (N-term, 1-384), ATPase binding domain, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp70, Co chaperone with DnaJ,. NCBI no.: NP_414555
- 分子量
- 41.6kDa (384aa)
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