GALT Protein (His tag)
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- 抗原 See all GALT 蛋白
- GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This GALT protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product GALT 蛋白
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- 说明
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Synonyms: Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.5) containing 0.2 M NaCl, 10% glycerol.
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
- 别名
- GALT (GALT 产品)
- 背景
- GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
- 分子量
- 45.9 kDa (403aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_000146
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