NAD Synthetase protein (His tag)
-
- 抗原
- NAD Synthetase
- 蛋白类型
- Recombinant
- 宿主
- Mycobacterium tuberculosis
-
资源
- 大肠杆菌(E. Coli)
- 标记
- His tag
- 原理
- NAD Synthetase (M. tuberculosis) (rec.) (His)
- 特异性
- NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
- 产品特性
- Protein. NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 20 mM TRIS-HCl, pH 7.8. Purity: >98 % (SDS-PAGE). NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate
- 纯度
- >98 % (SDS-PAGE)
- Biological Activity Comment
- ~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.
-
-
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. In 20 mM TRIS-HCl, pH 7.8.
- 注意事项
- After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.
- 储存条件
- -20 °C,-80 °C
- 储存方法
-
Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
-
- 抗原
- NAD Synthetase
- 背景
-
Alternate Names/Synonyms: NADE, Glutamine-dependent NAD(+) Synthetase, EC 6.3.5.1
Product Description: NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate
- UniProt
- P0A5L6
-