Flagellin Protein (FliC) (Mutant) (His tag)
-
- 抗原 See all Flagellin (FliC) 蛋白
- Flagellin (FliC)
- 蛋白类型
- Recombinant
- 产品特性
- Mutant
-
宿主
- 人, 小鼠
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This Flagellin protein is labelled with His tag.
- 原理
- Flagellin (TLR5 Mutant) (rec.)
- 特异性
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
- 交叉反应
- 人, 小鼠
- 产品特性
- The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and is fused at the N-terminus to a His-tag.
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.01EU/μg purified protein (LAL test).
- Biological Activity Comment
- Does not activate TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays.
- Top Product
- Discover our top product FliC 蛋白
-
Flagellin (FliC) (AA 2-498) protein (His tag)
FliC 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Flagellin (FliC) proteinFliC 宿主: Borrelia burgdorferi 宿主: 大肠杆菌（E. Coli） Recombinant ELISA, WB
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 浓度
- 0.1 mg/mL
- 缓冲液
- Contains PBS.
- 注意事项
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
- 有效期
- 6 months
-
- 抗原
- Flagellin (FliC)
- 别名
- Flagellin (FliC 产品)
- 背景
-
FliC (TLR5 Mutant)
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (TLR5 Mutant) (rec.) cannot be detected by human and mouse TLR5. It has not been tested on NLRC4.
- 途径
- Inflammasome
-
