Factor VII Protein (AA 315-433) (His tag)
F7
宿主: 大鼠
宿主: 大肠杆菌（E. Coli）
> 95 %
AcA, CC
Active
1 image
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- 抗原 See all Factor VII (F7) 蛋白
- Factor VII (F7) (Coagulation Factor VII (F7))
- 生物活性
- Active
- 产品特性
- AA 315-433
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Factor VII protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels-normally not exposed to the bloodstream. Upon vessel injury, tissue factor is exposed to the blood and circulating FVII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Tissue Factor (TF) has been identified as an interactor of FVII, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FVII and recombinant rat TF. Briefly, FVII were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TF-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-FVII pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of FVII and TF was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of FVII with TF.
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- Discover our top product F7 蛋白
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Coagulation Factor VII (F7) (AA 1-444) protein (GST tag)
F7 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Coagulation Factor VII (F7) (AA 103-212) protein (GST tag)F7 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 应用备注
- Isoelectric Point: 6.4
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- Factor VII (F7) (Coagulation Factor VII (F7))
- Abstract
- F7 产品
- 别名
- F7 Protein, wu:fb59f04 Protein, zgc:109870 Protein, SPCA Protein, AI114908 Protein, ATIII Protein, At-3 Protein, At3 Protein, LOC100223776 Protein, AI132620 Protein, Cf7 Protein, FVII Protein, mfVII Protein, f7 Protein, coagulation factor VII Protein, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Protein, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog Protein, F7 Protein, f7 Protein, Serpinc1 Protein, CpipJ_CPIJ009142 Protein, CpipJ_CPIJ010295 Protein, CpipJ_CPIJ020127 Protein, fa7 Protein, f7.S Protein
- 背景
- Alternative Names: FVII, SPCA, proconvertin, Cothromboplastin, Serum Prothrombin Conversation Accelerator, Stable Factor, Eptacog alfa
- 分子量
- 14kDa
- UniProt
- Q8K3U6
- 途径
- Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling
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