IL17 Receptor B Protein (AA 61-200) (His tag)
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- 抗原 See all IL17 Receptor B (IL17RB) 蛋白
- IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
- 生物活性
- Active
- 产品特性
- AA 61-200
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IL17 Receptor B protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 80 %
- Biological Activity Comment
- IL17RB (Interleukin-17 receptor B) is a receptor for the proinflammatory cytokines IL17B (Interleukin-17B) and IL17E (Interleukin-17E). This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RB with recombinant human IL17B. Briefly, IL17RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17B-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RB with IL17B was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17RB with IL17B.
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- Discover our top product IL17RB 蛋白
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Interleukin 17 Receptor B (IL17RB) (AA 18-292) protein (His tag)
IL17RB 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17 Receptor B (IL17RB) (AA 18-286) protein (His tag)IL17RB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17 Receptor B (IL17RB) (AA 317-494) protein (His-IF2DI Tag)IL17RB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 17 Receptor B (IL17RB) (AA 18-117) protein (GST tag)IL17RB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 17 Receptor B (IL17RB) (Active) protein (His tag)IL17RB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- 应用备注
- Isoelectric Point: 8.9
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
- Abstract
- IL17RB 产品
- 别名
- IL17RB Protein, CRL4 Protein, EVI27 Protein, IL17BR Protein, IL17RH1 Protein, Evi27 Protein, IL-17ER Protein, IL-17Rh1 Protein, Il17br Protein, interleukin 17 receptor B Protein, IL17RB Protein, Il17rb Protein
- 背景
- Alternative Names: IL17R-B, CRL4, EVI27, IL17BR, IL17RH1, Cytokine receptor-like 4, IL-17 receptor homolog 1
- 分子量
- 19kDa
- UniProt
- Q9NRM6
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