This Osteopontin protein is labelled with His tag.
应用范围
Activity Assay (AcA), Cell Culture (CC)
产品特性
Tag location: N-terminal His Tag
纯度
> 95 %
Biological Activity Comment
Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without various concentrations of OPN for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1. Sample Concentration of MCP-1 O.D. value Corrected (cell supernatant of MCF-7 cells) (ng/mL) stimulated with OPN (100ng/mL) 1.358 1.305 2.53 stimulated with OPN (200ng/mL) 1.177 1.124 2.15 stimulated with OPN (400ng/mL) 1.101 1.048 1.99 unstimulated 0.944 0.891 1.66 Table 1. MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN.
Alternative Names: SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin