FNDC5 Protein (AA 2-137) (His tag)
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- 抗原 See all FNDC5 蛋白
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- 生物活性
- Active
- 产品特性
- AA 2-137
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This FNDC5 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.
- Top Product
- Discover our top product FNDC5 蛋白
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- 应用备注
- Isoelectric Point: 6.2
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- 别名
- Fibronectin Type III Domain Containing Protein 5 (FNDC5) (FNDC5 产品)
- 别名
- FRCP2 Protein, irisin Protein, 1500001L03Rik Protein, AI836596 Protein, C87088 Protein, PeP Protein, Pxp Protein, fndc5 Protein, si:dkey-264p5.1 Protein, zgc:158684 Protein, fibronectin type III domain containing 5 Protein, fibronectin type III domain containing 5b Protein, FNDC5 Protein, Fndc5 Protein, fndc5b Protein
- 背景
- Alternative Names: FRCP2, Irisin, Fibronectin type III repeat-containing protein 2
- 分子量
- 18kDa
- UniProt
- Q8NAU1
- 途径
- Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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