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GARS Protein (AA 43-289) (His tag)
GARS
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 85 % by SDS - PAGE
SDS
产品细节
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抗原
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GARS
(Glycyl-tRNA Synthetase (GARS))
蛋白类型
Recombinant
产品特性
AA 43-289
宿主
All origins for GARS 蛋白
人
资源
All sources for GARS 蛋白
大肠杆菌(E. Coli)
标记
This GARS protein is labelled with His tag.
应用范围
SDS-PAGE (SDS)
序列
MGSSHHHHHH SSGLVPRGSH MGSPISLPAA ASRSSMDGAG AEEVLAPLRL AVRQQGDLVR KLKEDKAPQV DVDKAVAELK ARKRVLEAKE LALQPKDDIV DRAKMEDTLK RRFFYDQAFA IYGGVSGLYD FGPVGCALKN NIIQTWRQHF IQEEQILEID CTMLTPEPVL KTSGHVDKFA DFMVKDVKNG ECFRADHLLK AHLQKLMSDK KCSVEKKSEM ESVLAQLDNY GQQELADLFV NYNVKSPITG NDLSPPVSFN LMFKTFIGPG
纯度
> 85 % by SDS - PAGE
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
0.5 mg/mL
缓冲液
Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
储存条件
4 °C,-20 °C,-80 °C
储存方法
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
目标详细情况
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抗原
GARS
(Glycyl-tRNA Synthetase (GARS))
别名
GARS (GARS 产品 )
别名
CMT2D Protein, DSMAV Protein, GlyRS Protein, HMN5 Protein, SMAD1 Protein, Aat-gly Protein, CG6778 Protein, Dmel\\CG6778 Protein, GRS Protein, gars Protein, team Protein, RGD1559871 Protein, GENA202 Protein, Gena201 Protein, Nmf249 Protein, Sgrp23 Protein, GB13467 Protein, MGC79495 Protein, fb02f03 Protein, si:dkey-276i5.1 Protein, wu:fb02f03 Protein, NI36_08435 Protein, glycyl-tRNA synthetase Protein, Glycyl-tRNA synthetase Protein, glycine--tRNA ligase Protein, glycyl-tRNA synthetase L homeolog Protein, Glycine--tRNA ligase Protein, GARS Protein, GlyRS Protein, Gars Protein, LOC408392 Protein, gars.L Protein, gars Protein, LOC692991 Protein, glyQ Protein, TTHA0543 Protein, NI36_RS08405 Protein
背景
GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Recombinant human GARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
分子量
30.0 kDa (270aa), confirmed by MALDI-TOF
NCBI登录号
NP_002038
UniProt
P41250
途径
Ribonucleoside Biosynthetic Process
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GARS 蛋白
GARS Protein (AA 43-289) (His tag) (ABIN7280733)