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ZWILCH Protein (AA 1-591) (His tag)
ZWILCH
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 85 % by SDS - PAGE
SDS
产品细节
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抗原
See all ZWILCH 蛋白
ZWILCH
(Zwilch, Kinetochore Associated, Homolog (ZWILCH))
蛋白类型
Recombinant
产品特性
AA 1-591
宿主
人
资源
All sources for ZWILCH 蛋白
大肠杆菌(E. Coli)
标记
This ZWILCH protein is labelled with His tag.
应用范围
SDS-PAGE (SDS)
序列
MGSSHHHHHH SSGLVPRGSH MGSMWERLNC AAEDFYSRLL QKFNEEKKGI RKDPFLYEAD VQVQLISKGQ PNPLKNILNE NDIVFIVEKV PLEKEETSHI EELQSEETAI SDFSTGENVG PLALPVGKAR QLIGLYTMAH NPNMTHLKIN LPVTALPPLW VRCDSSDPEG TCWLGAELIT TNNSITGIVL YVVSCKADKN YSVNLENLKN LHKKRHHLST VTSKGFAQYE LFKSSALDDT ITASQTAIAL DISWSPVDEI LQIPPLSSTA TLNIKVESGE PRGPLNHLYR ELKFLLVLAD GLRTGVTEWL EPLEAKSAVE LVQEFLNDLN KLDGFGDSTK KDTEVETLKH DTAAVDRSVK RLFKVRSDLD FAEQLWCKMS SSVISYQDLV KCFTLIIQSL QRGDIQPWLH SGSNSLLSKL IHQSYHGTMD TVSLSGTIPV QMLLEIGLDK LKKDYISFFI GQELASLNHL EYFIAPSVDI QEQVYRVQKL HHILEILVSC MPFIKSQHEL LFSLTQICIK YYKQNPLDEQ HIFQLPVRPT AVKNLYQSEK PQKWRVEIYS GQKKIKTVWQ LSDSSPIDHL NFHKPDFSEL TLNGSLEERI FFTNMVTCSQ VHFK
纯度
> 85 % by SDS - PAGE
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
0.25 mg/mL
缓冲液
Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 20 % glycerol, 1 mM DTT
储存条件
4 °C,-20 °C,-80 °C
储存方法
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
目标详细情况
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抗原
ZWILCH
(Zwilch, Kinetochore Associated, Homolog (ZWILCH))
别名
ZWILCH (ZWILCH 产品 )
别名
CG18639 Protein, CG18729 Protein, Dmel\\CG18729 Protein, Zwilch Protein, hzwilch Protein, kntc1ap Protein, KNTC1AP Protein, hZwilch Protein, 2310031L18Rik Protein, si:ch211-242m18.4 Protein, wu:fb10f09 Protein, zwilch kinetochore protein Protein, CG18729 gene product from transcript CG18729-RA Protein, protein zwilch homolog Protein, Zwilch Protein, zwilch Protein, LOC100374963 Protein, ZWILCH Protein
背景
ZWILCH belongs to the ZWILCH family. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. It is required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Recombinant human ZWILCH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
分子量
69.6kDa(614aa)
NCBI登录号
NP_060445
UniProt
Q9H900
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