Streptavidin Protein (AA 37-159) (His tag)
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- 抗原 See all Streptavidin 蛋白
- Streptavidin
- 蛋白类型
- Recombinant
- 产品特性
- AA 37-159
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宿主
- Streptomyces
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Streptavidin protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSHMAEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP
- 纯度
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product Streptavidin 蛋白
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Streptavidin protein (Alkaline Phosphatase (AP))宿主: Streptomyces avidinii Recombinant WB, ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- Streptavidin
- Abstract
- Streptavidin 产品
- 背景
- Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 15.6kDa (148aa), confirmed by MALDI-TOF
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