MUG (AA 1-168) protein (His tag)
宿主: E. coli
宿主: 大肠杆菌（E. Coli）
Recombinant
> 90 % by SDS - PAGE
SDS
1 image
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- 抗原
- MUG
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-168
- 宿主
- E. coli
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资源
- 大肠杆菌（E. Coli）
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R
- 纯度
- > 90 % by SDS - PAGE
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 20 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- MUG
- 背景
- G/u mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of u:G>u:A, although the biological role remains unclear. The enzyme uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E.coli μg protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 21.1 kDa (191aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_417540
- UniProt
- P0A9H1
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