MEK1 Protein (pSer218, pSer222)
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- 抗原 See all MEK1 (MAP2K1) 蛋白
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
- 蛋白类型
- Recombinant
- 产品特性
- pSer218, pSer222
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宿主
- 人, 小鼠, 大鼠
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资源
- Insect cells (Sf9)
- 应用范围
- Western Blotting (WB)
- 纯化方法
- MEK1 pS218-pS222 is a recombinant protein containing a polyhistidine tag expressed in Sf9. This protein is expressed in the presense of constitutively active B-raf (V600E) leading to high levels of phosphorylation of MEK1 activation residues at position S218 and S222. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 95% pure.
- 过滤
- Sterile filtered
- Top Product
- Discover our top product MAP2K1 蛋白
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Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-58) protein (GST tag)
MAP2K1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 294-393) protein (GST tag)MAP2K1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) protein (GST tag,His tag)MAP2K1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) proteinMAP2K1 宿主: 人, 小鼠, 大鼠 宿主: Insect cells (Sf9) Recombinant WB
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) protein (Myc-DYKDDDDK Tag)MAP2K1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Application Note: MEK1 pS218-pS222 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-MEK1 in immunological assays. Activated MEK1 can be used in vitro to phosphorylate ERK protein. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Stabilizer: None
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
- 别名
- MEK1 (MAP2K1 产品)
- 别名
- Mek1 Protein, CFC3 Protein, MAPKK1 Protein, MEK1 Protein, MKK1 Protein, PRKMK1 Protein, MEKK1 Protein, Prkmk1 Protein, mek-2 Protein, si:ch211-242m18.2 Protein, wu:fj56a12 Protein, wu:fj61b01 Protein, zgc:56557 Protein, ATMEK1 Protein, F20B18.180 Protein, F20B18_180 Protein, MAP kinase/ ERK kinase 1 Protein, MITOGEN ACTIVATED PROTEIN KINASE KINASE Protein, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 Protein, NMAPKK Protein, MEK Protein, mitogen activated protein kinase kinase 1 Protein, mitogen-activated protein kinase kinase 1 Protein, mitogen-activated protein kinase kinase 1 L homeolog Protein, MAP kinase/ ERK kinase 1 Protein, Map2k1 Protein, MAP2K1 Protein, map2k1.L Protein, map2k1 Protein, MEK1 Protein
- UniProt
- Q02750
- 途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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