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- 抗原 See all Collagen Type V (COL5) products
- Collagen Type V (COL5) (Collagen, Type V (COL5))
- 蛋白类型
- Native
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宿主
- Cow
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资源
- 牛
- 应用范围
- ELISA, Immunohistochemistry (IHC), SDS-PAGE (SDS), Western Blotting (WB)
- Supplier Product No.
- 001-001-107
- Supplier
- Rockland
- 原理
- Bovine Collagen Type V
- 产品特性
- Extracellular Matrix Protein
- 纯化方法
- Bovine COLLAGEN Type V has been prepared from Bovine Placenta and is chromatographically and immunologically pure. This product reacts with anti-Collagen Type V. Reaction with Rockland's anti-Collagen I, II, III, IV or VI is negligible.
- 过滤
- Sterile filtered
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Collagen, Type V (COL5) protein
COL5 宿主: 人 宿主: Human Native ELISA, IHC, SDS, WB
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- 应用备注
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Immunohistochemistry Dilution: 1:100-1:500
Application Note: Bovine COLLAGEN Type V has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.5 M Acetic Acid
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 6 months
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- 抗原
- Collagen Type V (COL5) (Collagen, Type V (COL5))
- 别名
- Collagen Type V (COL5 产品)
- 背景
- Background: In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6 % of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.
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