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Collagen IV 蛋白

Rockland 009-001-106 COL4 宿主: 人 宿主: Human Native ELISA, IHC, SDS, WB
Rockland
产品编号 ABIN5624596
Supplier Product No.: 009-001-106
发货至: 中国
  • 抗原 See all Collagen IV (COL4) 蛋白
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    蛋白类型
    Native
    宿主
    • 1
    • 1
    • 1
    资源
    • 1
    • 1
    • 1
    Human
    应用范围
    ELISA, Immunohistochemistry (IHC), SDS-PAGE (SDS), Western Blotting (WB)
    Supplier Product No.
    009-001-106
    Supplier
    Rockland
    原理
    Human Collagen Type IV
    产品特性
    Type IV collagen, collagen 4, human collagen, Collagen alpha-4 (IV) chain
    纯化方法
    This product has been prepared from human placenta and is chromatographically and immunologically pure.  This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  This product reacts with anti-Collagen Type IV.  Reaction with Rockland's anti-Collagen I, II, III, V or VI is negligible.
    过滤
    Sterile filtered
    Top Product
    Discover our top product COL4 蛋白
  • 应用备注

    Immunohistochemistry Dilution: 1:100-1:500

    Application Note: Collagen type IV has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

    Western Blot Dilution: 1:1000

    ELISA Dilution: 1:10,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.5 M Acetic Acid

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    6 months
  • 抗原
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    别名
    COLLAGEN IV (COL4 产品)
    背景
    Background: Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.
    UniProt
    P02462
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