Collagen IV 蛋白
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- 抗原 See all Collagen IV (COL4) 蛋白
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- 蛋白类型
- Native
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宿主
- 人
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资源
- Human
- 应用范围
- ELISA, Immunohistochemistry (IHC), SDS-PAGE (SDS), Western Blotting (WB)
- Supplier Product No.
- 009-001-106
- Supplier
- Rockland
- 原理
- Human Collagen Type IV
- 产品特性
- Type IV collagen, collagen 4, human collagen, Collagen alpha-4 (IV) chain
- 纯化方法
- This product has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type IV. Reaction with Rockland's anti-Collagen I, II, III, V or VI is negligible.
- 过滤
- Sterile filtered
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Collagen, Type IV (COL4) protein
COL4 宿主: Cow 宿主: 牛 Native ELISA, IHC, SDS, WB
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- 应用备注
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Immunohistochemistry Dilution: 1:100-1:500
Application Note: Collagen type IV has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.5 M Acetic Acid
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 6 months
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- 抗原
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- 别名
- COLLAGEN IV (COL4 产品)
- 背景
- Background: Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.
- UniProt
- P02462
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