ERK1 蛋白
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- 抗原 See all ERK1 (MAPK3) 蛋白
- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-gq8
- Supplier
- Rockland
- 原理
- ERK1 human recombinant protein
- 产品特性
- MAPK3, ERK, ERK1, PRKM3, ERK-1 recombinant protein
- 纯化方法
- ERK1 is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 90% pure.
- 过滤
- Sterile filtered
- Top Product
- Discover our top product MAPK3 蛋白
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Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-379) protein (His tag)
MAPK3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) protein (GST tag)MAPK3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-379) protein (GST tag)MAPK3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase 3 (MAPK3) (double Mutant) proteinMAPK3 宿主: 人 宿主: Insect cells (Sf9) Recombinant WB, SDS
Mitogen-Activated Protein Kinase 3 (MAPK3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)MAPK3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Application Note: Human ERK1 recombinant protein has been tested in SDS-Page and is suitable as a control for polyclonal or monoclonal anti-ERK1 in immunological assays. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
Other: Dot Blot
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
- 别名
- MAPK3 (MAPK3 产品)
- 背景
- Background: ERK1 recombinant protein was produced in Sf9 cells. Mitogen activated protein kinase 3, also known as MAPK3, ERK, or ERK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84 % identical in sequence and share common functionality in cells. Recombinant ERK1 protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
- UniProt
- P27361
- 途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
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