AKT2 蛋白
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- 抗原 See all AKT2 蛋白
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-p23
- Supplier
- Rockland
- 纯化方法
- Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2, Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
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V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein
AKT2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant WB
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-123) protein (His-IF2DI Tag)AKT2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (GST tag,His tag)AKT2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Active) protein (Myc-DYKDDDDK Tag)AKT2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
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- 应用备注
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Application Note: AKT2 is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.09 mg/mL
- 缓冲液
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Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol
Stabilizer: 10 % (v/v) Glycerol
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
- Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 12 months
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- 抗原
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 别名
- AKT2 (AKT2 产品)
- 基因ID
- 208
- UniProt
- P31751
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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