PARP1 Protein (Glu988Lys-Mutant) (HA tag,His tag)
-
- 抗原 See all PARP1 蛋白
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Glu988Lys-Mutant
-
宿主
- 人
-
资源
- Insect cells (Sf21)
- 标记
- This PARP1 protein is labelled with HA tag,His tag.
- 应用范围
- SDS-PAGE (SDS)
- 交叉反应
- 人
- 产品特性
- Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag.
- 纯度
- >95 % (SDS-PAGE)
- Top Product
- Discover our top product PARP1 蛋白
-
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Active) protein
PARP1 宿主: 人 宿主: Baculovirus Recombinant > 95 % WB, ELISA, PAR Active
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Internal Region) proteinPARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Ct
Poly (ADP-Ribose) Polymerase 1 (PARP1) proteinPARP1 宿主: 人 宿主: Baculovirus Recombinant > 95 % WB, ELISA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (N-Term) proteinPARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Ct
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 787-1014) protein (His-IF2DI Tag)PARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 1-100) protein (GST tag)PARP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Active) protein (His tag)PARP1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
0.5% of wild type PARP-1.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride and 50 mM imidazole, 0.2 % NP-40 and 10 % glycerol.
- 储存条件
- -20 °C,-80 °C
- 储存方法
-
Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C. - 有效期
- 6 months
-
- 抗原
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- 别名
- PARP-1 [ARTD1] (PARP1 产品)
- 别名
- ADPRT Protein, ADPRT 1 Protein, ADPRT1 Protein, ARTD1 Protein, PARP Protein, PARP-1 Protein, PPOL Protein, pADPRT-1 Protein, Adprt Protein, Parp-1 Protein, 5830444G22Rik Protein, AI893648 Protein, Adprp Protein, Adprt1 Protein, C80510 Protein, parp-1 Protein, sPARP-1 Protein, BEST:LD21673 Protein, CG17685 Protein, CG17696 Protein, CG17718 Protein, CG40411 Protein, D.PARP Protein, Dm.pARTa Protein, Dmel\\CG40411 Protein, LD21673.3prime Protein, PARP1 Protein, dPARP Protein, parp Protein, adprt1 Protein, padprt-1 Protein, ppol Protein, si:dkey-206f10.3 Protein, wu:fc60f12 Protein, zgc:110092 Protein, poly(ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase family, member 1 Protein, Poly-(ADP-ribose) polymerase Protein, poly(ADP-ribose) polymerase 1 L homeolog Protein, PARP1 Protein, Parp1 Protein, Parp Protein, parp1.L Protein, parp1 Protein
- 背景
- PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound.
- UniProt
- P09874
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
-
