GLUL 蛋白
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- 抗原 See all GLUL 蛋白
- GLUL (Glutamate-Ammonia Ligase (GLUL))
- 适用
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- 宿主
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- 应用范围
- Western Blotting (WB)
- 品牌
- SERVA®
- 纯化方法
- affinity purified
- Top Product
- Discover our top product GLUL 蛋白
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Glutamate-Ammonia Ligase (GLUL) (AA 228-373) protein (His-IF2DI Tag)
GLUL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Glutamate-Ammonia Ligase (GLUL) (AA 1-373) protein (His tag)GLUL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Glutamate-Ammonia Ligase (GLUL) (AA 274-373) protein (GST tag)GLUL 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Glutamate-Ammonia Ligase (GLUL) (AA 1-373) protein (GST tag)GLUL 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Glutamate-Ammonia Ligase (GLUL) (AA 1-373) proteinGLUL 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS
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- 应用备注
- Standard curve: 3 loads are recommended (0.5, 2 and 4i1/4l).For most applications a sample load of 0.2i1/4g of chlorophyll will give a GlnA signal in this range.Positive control: a 2i1/4l load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10 % glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
- 说明
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Concentration: after adding 225 μl of milliQ water final concentration of the standard is 0.20 pmol/μlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 225 μL of milliQ water
- 缓冲液
- Glycerol 10 %, Tris Base 141 mM, Tris HCl 106 mM, LDS 2 %, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1 mg/mL PefaBloc protease inhibitor (Roche), 50 mM DTT. This standard is ready-to-load and does not require any additions or heating.
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- 抗原
- GLUL (Glutamate-Ammonia Ligase (GLUL))
- 别名
- Glutamine synthetase (GlnA) (GLUL 产品)
- 别名
- GLNS Protein, GS Protein, PIG43 Protein, PIG59 Protein, Glns Protein, glns Protein, glul Protein, pig43 Protein, pig59 Protein, xgs Protein, p42 Protein, MGC64262 Protein, cb195 Protein, cb757 Protein, etID49849.2 Protein, fb39g09 Protein, fd19d09 Protein, gso1 Protein, sb:cb195 Protein, wu:fb39g09 Protein, wu:fd19d09 Protein, fb11d08 Protein, gso2 Protein, hm:zehn1949 Protein, im:7153900 Protein, wu:fb11d08 Protein, glutamate-ammonia ligase Protein, glutamate-ammonia ligase (glutamine synthetase) Protein, type I glutamate--ammonia ligase Protein, glutamate-ammonia ligase L homeolog Protein, glutamate-ammonia ligase (glutamine synthase) Protein, glutamate-ammonia ligase (glutamine synthase) a Protein, glutamate-ammonia ligase (glutamine synthase) b Protein, GLUL Protein, Glul Protein, AMF_RS09530 Protein, glul.L Protein, glul Protein, CNA04370 Protein, glula Protein, glulb Protein
- 背景
- Glutamine synthetase (GlnA) is the key enzyme in the incorporation of mineral nitrogen into glutamine.This product is a recombinant GlnA protein standard, source Synechocystis strain PCC 6803.
- 分子量
- in most gel systems GlnA migrates around 53 kDa
- 途径
- Positive Regulation of Peptide Hormone Secretion
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