Protein L Protein (His tag)
This Recombinant Protein L protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 1 publication.
(1 reference)Quick Overview for Protein L Protein (His tag) (ABIN2181671)
抗原
See all Protein L 蛋白
Protein L
蛋白类型
Recombinant
宿主
Peptostreptococcus magnus
资源
大肠杆菌（E. Coli）
纯度
>95 % as determined by SDS-PAGE.
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标记
- This Protein L protein is labelled with His tag.
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品牌
- MABSol®
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产品特性
- Recombinant Protein L Protein is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 36-38 kDa polypeptide .Protein L Protein can bind to VL-Kappa.
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内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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Protein L (AA 106-470) (Active) protein (His tag,AVI tag,Biotin)
宿主: Peptostreptococcus magnus 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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缓冲液
- 100 mM NaH2PO4, pH 8.5
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
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: "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum." in: Electrophoresis, Vol. 38, Issue 22-23, pp. 2909-2913, (2017) (PubMed).
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: "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum." in: Electrophoresis, Vol. 38, Issue 22-23, pp. 2909-2913, (2017) (PubMed).
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- Protein L
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背景
- Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
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基因ID
- 17
抗原
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