ASIC3 Protein (AA 1-530) (Strep Tag)
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- 抗原 See all ASIC3 (ACCN3) 蛋白
- ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-530
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This ASIC3 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MKPPSGLEEA QRRQASDIRV FANSCTMHGL GHIFGPGGLT LRRGLWATAV LLSLAAFLYQ VAERVRYYGE FHHKTTLDER ESHQLTFPAV TLCNINPLRR SRLTPNDLHW AGTALLGLDP AEHAAYLRAL GQPPAPPGFM PSPTFDMAQL YARAGHSLED MLLDCRYRGQ PCGPENFTVI FTRMGQCYTF NSGAQGAELL TTPKGGAGNG LEIMLDVQQE EYLPIWKDME ETPFEVGIRV QIHGQEEPPA IDQLGFGAAP GHQTFVSCQQ QQLSFLPPPW GDCNTASVDP DFDPEPSDPL GSPSSSPPYS LIGCRLACES RYVARKCGCR MMHMPGNSPV CSPQQYKDCA SPALDAMLRK DTCVCPNPCA TTRYAKELSM VRIPSRASAR YLARKYNRSE TYITENVLVL DIFFEALNYE AVEQKAAYEV SELLGDIGGQ MGLFIGASLL TILEILDYLC EVFQDRVLGY FWNRRSSQRR SGNTLLQEEL NGHRTHVPHL SLGPRPPTAP SAVTKTLAAS HRTCYLVTRL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ACCN3 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))
- 别名
- Asic3 (ACCN3 产品)
- 别名
- ACCN3 Protein, Accn3 Protein, DRASIC Protein, SLNAC1 Protein, TNaC1 Protein, AW742291 Protein, TNAC1 Protein, amiloride-sensitive cation channel 3 Protein, acid sensing ion channel subunit 3 Protein, acid-sensing (proton-gated) ion channel 3 Protein, ACCN3 Protein, ASIC3 Protein, Asic3 Protein
- 背景
- Acid-sensing ion channel 3 (ASIC3) (Amiloride-sensitive cation channel 3) (Dorsal root ASIC) (DRASIC),FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. {ECO:0000269|PubMed:11754838, ECO:0000269|PubMed:12060708, ECO:0000269|PubMed:14659506}.
- 分子量
- 58.7 kDa
- UniProt
- Q6X1Y6
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