CX3CR1 Protein (AA 1-354) (Strep Tag)
-
- 抗原 See all CX3CR1 蛋白
- CX3CR1 (Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-354
-
宿主
- 小鼠
-
资源
- Tobacco (Nicotiana tabacum)
- 标记
- This CX3CR1 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
-
MSTSFPELDL ENFEYDDSAE ACYLGDIVAF GTIFLSVFYA LVFTFGLVGN LLVVLALTNS RKPKSITDIY LLNLALSDLL FVATLPFWTH YLISHEGLHN AMCKLTTAFF FIGFFGGIFF ITVISIDRYL AIVLAANSMN NRTVQHGVTI SLGVWAAAIL VASPQFMFTK RKDNECLGDY PEVLQEMWPV LRNSEVNILG FALPLLIMSF CYFRIIQTLF SCKNRKKARA VRLILLVVFA FFLFWTPYNI MIFLETLKFY NFFPSCDMKR DLRLALSVTE TVAFSHCCLN PFIYAFAGEK FRRYLGHLYR KCLAVLCGHP VHTGFSPESQ RSRQDSILSS FTHYTSEGDG SLLL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product CX3CR1 蛋白
-
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- CX3CR1 (Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1))
- 别名
- Cx3cr1 (CX3CR1 产品)
- 别名
- CCRL1 Protein, CMKBRL1 Protein, CMKDR1 Protein, GPR13 Protein, GPRV28 Protein, V28 Protein, Rbs11 Protein, C-X3-C motif chemokine receptor 1 Protein, chemokine (C-X3-C motif) receptor 1 Protein, CX3C chemokine receptor 1 Protein, CX3CR1 Protein, Cx3cr1 Protein, LOC100559882 Protein
- 背景
- CX3C chemokine receptor 1 (C-X3-C CKR-1) (CX3CR1) (mCX3CR1) (Fractalkine receptor),FUNCTION: Receptor for the C-X3-C chemokine fractalkine (CX3CL1) present on many early leukocyte cells, CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed:9918795, PubMed:10187784). CX3CR1-CX3CL1 signaling mediates cell migratory functions (PubMed:11544273, PubMed:12871640, PubMed:16675847, PubMed:18322241). Responsible for the recruitment of natural killer (NK) cells to inflamed tissues (PubMed:11544273, PubMed:16675847). Acts as a regulator of inflammation process leading to atherogenesis by mediating macrophage and monocyte recruitment to inflamed atherosclerotic plaques, promoting cell survival (PubMed:12569158, PubMed:18971423). Involved in airway inflammation by promoting interleukin 2-producing T helper (Th2) cell survival in inflamed lung (PubMed:21037587). Involved in the migration of circulating monocytes to non-inflamed tissues, where they differentiate into macrophages and dendritic cells (PubMed:12871640). Acts as a negative regulator of angiogenesis, probably by promoting macrophage chemotaxis (PubMed:18322241). Plays a key role in brain microglia by regulating inflammatory response in the central nervous system (CNS) and regulating synapse maturation (PubMed:16732273, PubMed:21778362, PubMed:24487234). Required to restrain the microglial inflammatory response in the CNS and the resulting parenchymal damage in response to pathological stimuli (PubMed:16732273). Involved in brain development by participating in synaptic pruning, a natural process during which brain microglia eliminates extra synapses during postnatal development (PubMed:21778362). Synaptic pruning by microglia is required to promote the maturation of circuit connectivity during brain development (PubMed:24487234). Acts as an important regulator of the gut microbiota by controlling immunity to intestinal bacteria and fungi (PubMed:15653504, PubMed:29326275). Expressed in lamina propria dendritic cells in the small intestine, which form transepithelial dendrites capable of taking up bacteria in order to provide defense against pathogenic bacteria (PubMed:15653504). Required to initiate innate and adaptive immune responses against dissemination of commensal fungi (mycobiota) component of the gut: expressed in mononuclear phagocytes (MNPs) and acts by promoting induction of antifungal IgG antibodies response to confer protection against disseminated C.albicans or C.auris infection (PubMed:29326275, PubMed:33548172). Also acts as a receptor for C-C motif chemokine CCL26, inducing cell chemotaxis (By similarity). {ECO:0000250|UniProtKB:P49238, ECO:0000269|PubMed:10187784, ECO:0000269|PubMed:11544273, ECO:0000269|PubMed:12569158, ECO:0000269|PubMed:12871640, ECO:0000269|PubMed:15653504, ECO:0000269|PubMed:16675847, ECO:0000269|PubMed:16732273, ECO:0000269|PubMed:18322241, ECO:0000269|PubMed:18971423, ECO:0000269|PubMed:21037587, ECO:0000269|PubMed:21778362, ECO:0000269|PubMed:24487234, ECO:0000269|PubMed:29326275, ECO:0000269|PubMed:33548172, ECO:0000269|PubMed:9918795}.
- 分子量
- 40.3 kDa
- UniProt
- Q9Z0D9
- 途径
- Cellular Response to Molecule of Bacterial Origin
-