BRD4 Protein (AA 1-1400) (Strep Tag)
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- 抗原 See all BRD4 蛋白
- BRD4 (Bromodomain Containing 4 (BRD4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1400
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This BRD4 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MSTESGPGTR LRNLPVMGDG LETSQMSTTQ AQAQPQPANA ASTNPPPPET SNPNKPKRQT NQLQYLLRVV LKTLWKHQFA WPFQQPVDAV KLNLPDYYKI IKTPMDMGTI KKRLENNYYW NAQECIQDFN TMFTNCYIYN KPGDDIVLMA EALEKLFLQK INELPTEETE IMIVQAKGRG RGRKETGAAK PGVSTVPNTT QASTSPQTQT PQQNPPPPVQ ATTHPFPAVT PDLIAQPPVM TMVPPQPLQT PSPVPPQPPP PPAPVPQPVQ SHPPIIATTP QPVKTKKGVK RKADTTTPTT IDPIHEPPSL APEPKTAKLG PRRESSRPVK PPKKDVPDSQ QHPGPEKSSK ISEQLKCCSG ILKEMFAKKH AAYAWPFYKP VDVEALGLHD YCDIIKHPMD MSTIKSKLES REYRDAQEFG ADVRLMFSNC YKYNPPDHEV VAMARKLQDV FEMRFAKMPD EPEEPVVTVS SPAVPPPTKV VAPPSSSDSS SDSSSDSDSS TDDSEEERAQ RLAELQEQLK AVHEQLAALS QPQQNKPKKK EKDKKEKKKE KHKKKEEVEE NKKSKTKELP PKKTKKNNSS NSNVSKKEPV PTKTKPPPTY ESEEEDKCKP MSYEEKRQLS LDINKLPGEK LGRVVHIIQS REPSLKNSNP DEIEIDFETL KPSTLRELER YVTSCLRKKR KPQAEKVDVI AGSSKMKGFS SSESESTSES SSSDSEDSET EMAPKSKKKG HTGRDQKKHH HHHHPQMQPA PAPVPQQPPP PPQQPPPPPP PQQQQQQPPP PPPPPSMPQQ TAPAMKSSPP PFITAQVPVL EPQLPGSVFD PIGHFTQPIL HLPQPELPPH LPQPPEHSTP PHLNQHAVVS PPALHNALPQ QPSRPSNRAA ALPPKPTRPP AVSPALAQPP LLPQPPMAQP PQVLLEDEEP PAPPLTSMQM QLYLQQLQKV QPPTPLLPSV KVQSQPPPPL PPPPHPSVQQ QQLQPQPPPP PPPQPQPPPQ QQHQPPPRPV HLPSMPFSAH IQQPPPPPGQ QPTHPPPGQQ PPPPQPAKPQ QVIQHHPSPR HHKSDPYSAG HLREAPSPLM IHSPQMPQFQ SLTHQSPPQQ NVQPKKQVKG RAEPQPPGPV MGQGQGCPPA SPAAVPMLSQ ELRPPSVVQP QPLVVVKEEK IHSPIIRSEP FSTSLRPEPP KHPENIKAPV HLPQRPEMKP VDIGRPVIRP PEQSAPPPGA PDKDKQKQEP KTPVAPKKDL KIKNMGSWAS LVQKHPTTPS STAKSSSDSF EHFRRAAREK EEREKALKAQ AEHAEKEKER LRQERMRSRE DEDALEQARR AHEEARRRQE QQQQQQQQRQ EQQQQQQQAA AVAAASAPQA QSSQPQSMLD QQRELARKRE QERRRREAMA ATIDMNFQSD LLSIFEENLF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product BRD4 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- BRD4 (Bromodomain Containing 4 (BRD4))
- 别名
- Brd4 (BRD4 产品)
- 别名
- brd4-b Protein, BRD4 Protein, fb18g03 Protein, wu:fb18g03 Protein, CAP Protein, HUNK1 Protein, HUNKI Protein, MCAP Protein, Brd5 Protein, WI-11513 Protein, bromodomain containing 4 L homeolog Protein, bromodomain containing 4 Protein, bromodomain-containing protein 4 Protein, brd4.L Protein, BRD4 Protein, brd4 Protein, LOC100409351 Protein, Brd4 Protein
- 背景
- Bromodomain-containing protein 4 (Mitotic chromosome-associated protein) (MCAP),FUNCTION: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed:10938129). During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (By similarity). Regulates differentiation of naive CD4(+) T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters (PubMed:28262505). Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II (PubMed:16109376). According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II, these data however need additional evidences in vivo. In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (By similarity). {ECO:0000250|UniProtKB:O60885, ECO:0000269|PubMed:10938129, ECO:0000269|PubMed:16109376, ECO:0000269|PubMed:28262505}.
- 分子量
- 155.9 kDa
- UniProt
- Q9ESU6
- 途径
- Chromatin Binding, SARS-CoV-2 Protein Interactome
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