STXBP5 Protein (AA 1-1152) (Strep Tag)
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- 抗原 See all STXBP5 蛋白
- STXBP5 (Syntaxin Binding Protein 5 (Tomosyn) (STXBP5))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1152
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This STXBP5 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC KTVRHGFPYQ PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA AVIQLQFLIN EGALVSALAD DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH LPFQSKWLYV GTERGNIHIV NVESFTLSGY VIMWNKAIEL SSKAHPGPVV HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA IHSVAWHHEG KQFICSHSDG TLTIWNVRSP AKPVQTITPH GKQLKDGKKP EPCKPILKVE LKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT LCETPYPNDF QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV TCCEYFADCP VDLIPALYSV GARQKRQGYS KKEWPINGGN WGLGAQSYPE IIITGHADGS VKFWDASAIT LQVLYKLKTS KVFEKSRNKD DRQNTDIVDE DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVLTEV IPMLEVRLLY EINDVDTPEG EQPPPLSTPV GSSNPQPIPP QSHPSTSSSS SDGLRDNVPC LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNC NGIAMVDYLQ KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI TEGTVVPEDR CKSPTSGSSS PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAN DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR SSSVTSIDKE SRETISALHF CETLTRKADS SPSPCLWVGT TVGTAFVITL NLPPGPEQRL LQPVIVSPSG TILRLKGAIL RMAFLDATGC LMSPAYEPWK EHNVAEEKDE KEKLKKRRPV SVSPSSSQEI SENQYAVICS EKQAKVMSLP TQSCAYKQNI TETSFVLRGD IVALSNSVCL ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA NNGQALYLVS PTEIQRLTYS QETCENLQEM LGELFTPVET PEAPNRGFFK GLFGGGAQSL DREELFGESS SGKASRSLAQ HIPGPGGIEG VKGAASGVVG ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM MLKYKDKKWY QF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product STXBP5 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- STXBP5 (Syntaxin Binding Protein 5 (Tomosyn) (STXBP5))
- 别名
- Stxbp5 (STXBP5 产品)
- 别名
- LGL3 Protein, LLGL3 Protein, Nbla04300 Protein, 0710001E20Rik Protein, 4930565N16Rik Protein, AW742610 Protein, mKIAA1006 Protein, syntaxin binding protein 5 Protein, syntaxin binding protein 5 (tomosyn) Protein, STXBP5 Protein, Stxbp5 Protein
- 背景
- Syntaxin-binding protein 5 (Lethal(2) giant larvae protein homolog 3) (Tomosyn-1),FUNCTION: Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release (By similarity). Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding. Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane. {ECO:0000250, ECO:0000269|PubMed:12832401}.
- 分子量
- 127.7 kDa
- UniProt
- Q8K400
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