AKNA Protein (AA 1-1404) (Strep Tag)
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- 抗原 See all AKNA 蛋白
- AKNA (AT-Hook Transcription Factor (AKNA))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1404
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This AKNA protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MASSGAKAQW VGPSLGQGPR RRRWAWAEEQ DTDGRRDQGW GNSQSLPEAP SPELLEDFRR AQEHLPPLEW DPDMQDSEES SGEETEADDA SSPEGSTVPL PWLSRSNQQL DMSEEELDEA SGRPEVDLAG ESCTELECED QGDSSPPPPG QGPAKGWVTF IKQGSNYRPS EHLEAQPSVE HSRTKSWSSG TVSLRQPSDS LGSTWEGDTE VPQPSILPKA LPQSPCHNFP HPGDRNGGDV APATPTEFRD SLAAPAQNAE CSAGTWGEET TSLPSSRPED QTWKRTKTSP KPLPSRFTGS VSPLSTRLGA IKKVVPQHKQ GATLAGHSSS QAPKYGRGRR LNYPLPDFSK VGPRVRFPKD ENYRPPKSRG HNRQQGSTRP LIFKSPAEIV RDVLLSSGEA SLAKESSLAH TITRVPQEFQ TPEQATELVH QLQEDYHKLL TKYAEAENTI DQLRLGAKVH LYSDPPQPSQ SFCSGSMPQG SKVLSFSIPQ PRVAEWWPDP AQDPQASEAT GWPFPRTDLS PSSSPGVATP GRLPQSQGIA TDQPSTGQTQ ALTSQASRLL AKVQSFEELV LAGHLPPQDQ IKSLEQLRAA HMALEAEYLQ ACREELLDPQ LDASQGSPRT LNLCRELEAE IYHLGQRLEE LQDHMDQTQR ETEPCRPDLQ DSTPTMSFLP QSAHLSMPSG PVSLPDGQTY QEPATTTTTS PGSSCTLPIN KKLSLSIKTE ESPRGLPVPL RDRTLQVEQD FHGLLERYLS VKSLPEALRD EDEDDLEEEE EEQDHQGPLE VDSPATAPGK TEAVRVPPGE RPTQAEESHR DATQEDEEQM GPMKSPDFRP SMARDTYTPV LDTAEVAQRG TKAMVSHQSS LTSLEESRPS ELLPRKALLR AGGPHTEEPW MVSPETDSGF VGSETSIVSP FTQTPEHRLS HVSTSGPSAQ HLTASVPGDR TSHPKARGLM VPRRATETGI PRSRTQQHFS SLSSPGRGAQ SCHLEETSVA KIAVPRSEFK RQKQISKQLL PSGRTSPDSA PAPTAASTPH GSAESTANLL LNRTERDQAI KDLQAEVSRL RLQLEDSLHR PHPDGPACVA SAFNHSTQTQ EKLGSSPSWG PHYGSKSTER LSREPNGVEP AEPMGRRRAR SSSVPRDVPR LYLSSESESP APRLSSEKSR TFEEHPEAAQ WGTRPQSSSK RRERVSFRGQ YTGQEYHILS PKAILKDSGT PSCPHCHPIR TQDTGSAVSR DTTRGSSAAD TLRCALCGEV KSSAEADGSS SGPSEKNTPK KPSTPILKRK NRQTGSPVRM APGLWYLAAA PPAPAPPALA YISSAPIMPY LPPTVYYAAP APTSAQTASP QPARGPRRTR HSVQLGLNDL EELQAALREA AQAAENVRST TRQLSRSLSA DLRHARSLRG SCLF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product AKNA 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- AKNA (AT-Hook Transcription Factor (AKNA))
- 别名
- Akna (AKNA 产品)
- 别名
- CASKIN1 Protein, AI597013 Protein, mKIAA1968 Protein, AT-hook transcription factor Protein, AT-hook-containing transcription factor Protein, Akna Protein, AKNA Protein, LOC539274 Protein
- 背景
- Microtubule organization protein AKNA (AT-hook-containing transcription factor),FUNCTION: Centrosomal protein that plays a key role in cell delamination by regulating microtubule organization (PubMed:30787442). Required for the delamination and retention of neural stem cells from the subventricular zone during neurogenesis (PubMed:30787442). Also regulates the epithelial-to-mesenchymal transition in other epithelial cells (PubMed:30787442). Acts by increasing centrosomal microtubule nucleation and recruiting nucleation factors and minus-end stabilizers, thereby destabilizing microtubules at the adherens junctions and mediating constriction of the apical endfoot (PubMed:30787442). In addition, may also act as a transcription factor that specifically activates the expression of the CD40 receptor and its ligand CD40L/CD154, two cell surface molecules on lymphocytes that are critical for antigen-dependent-B-cell development (By similarity). Binds to A/T-rich promoters (By similarity). It is unclear how it can both act as a microtubule organizer and as a transcription factor, additional evidences are required to reconcile these two apparently contradictory functions (Probable). {ECO:0000250|UniProtKB:Q7Z591, ECO:0000269|PubMed:30787442, ECO:0000305}.
- 分子量
- 153.1 kDa
- UniProt
- Q80VW7
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