BIRC2 Protein (AA 1-612) (Strep Tag)
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- 抗原 See all BIRC2 蛋白
- BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-612
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This BIRC2 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MDKTVSQRLG QGTLHQKLKR IMEKSTILSN WTKESEEKMK FDFSCELYRM STYSAFPRGV PVSERSLARA GFYYTGVNDK VKCFCCGLML DNWKQGDSPV EKHRQFYPSC SFVQTLLSAS LQSPSKNMSP VKSRFAHSSP LERGGIHSNL CSSPLNSRAV EDFSSRMDPC SYAMSTEEAR FLTYSMWPLS FLSPAELARA GFYYIGPGDR VACFACGGKL SNWEPKDDAM SEHRRHFPHC PFLENTSETQ RFSISNLSMQ THSARLRTFL YWPPSVPVQP EQLASAGFYY VDRNDDVKCF CCDGGLRCWE PGDDPWIEHA KWFPRCEFLI RMKGQEFVDE IQARYPHLLE QLLSTSDTPG EENADPTETV VHFGPGESSE DVVMMSTPVV KAALEMGFSR SLVRQTVQRQ ILATGENYRT VNDIVSVLLN AEDERREEEK ERQTEEMASG DLSLIRKNRM ALFQQLTHVL PILDNLLEAS VITKQEHDII RQKTQIPLQA RELIDTVLVK GNAAANIFKN SLKEIDSTLY ENLFVEKNMK YIPTEDVSGL SLEEQLRRLQ EERTCKVCMD REVSIVFIPC GHLVVCQECA PSLRKCPICR GTIKGTVRTF LS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product BIRC2 蛋白
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))
- 别名
- Birc2 (BIRC2 产品)
- 别名
- API1 Protein, HIAP2 Protein, Hiap-2 Protein, MIHB Protein, RNF48 Protein, c-IAP1 Protein, cIAP1 Protein, Api2 Protein, rIAP1 Protein, IAP1 Protein, ITA Protein, AW146227 Protein, Api1 Protein, Birc3 Protein, HIAP1 Protein, IAP2 Protein, MIAP1 Protein, MIAP2 Protein, MIHC Protein, cIAP2 Protein, mcIAP1 Protein, ciap-1 Protein, birc3 Protein, api1 Protein, birc2 Protein, c-iap1 Protein, ciap1 Protein, hiap-2 Protein, hiap2 Protein, mihb Protein, rnf48 Protein, baculoviral IAP repeat containing 2 Protein, baculoviral IAP repeat-containing 2 Protein, baculoviral IAP repeat containing 2 L homeolog Protein, BIRC2 Protein, Birc2 Protein, birc2 Protein, birc2.L Protein
- 背景
- Baculoviral IAP repeat-containing protein 2 (EC 2.3.2.27) (Cellular inhibitor of apoptosis 1) (C-IAP1) (Inhibitor of apoptosis protein 2) (mIAP2) (RING-type E3 ubiquitin transferase BIRC2),FUNCTION: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle. {ECO:0000269|PubMed:18621737}.
- 分子量
- 69.7 kDa
- UniProt
- Q62210
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades
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