PHF12 Protein (AA 1-1003) (Strep Tag)
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- 抗原 See all PHF12 products
- PHF12 (PHD Finger Protein 12 (PHF12))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1003
- 宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This PHF12 protein is labelled with Strep Tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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MWEKMETKTI VYDLDTSGGL MEQIQALLAP PKTDEAEKRS RKPEKESRRS GRATNHDSCD SCKEGGDLLC CDHCPAAFHL QCCNPPLSEE MLPPGEWMCH RCTVRRKKRE QKKELGHVNG LVDKSSKRTT SPSSDTDLLD RPASKTELKA IAHARILERR ASRPGTPTSN ASTETPTSEH NDVDEDIVDV DEEPVAAEPD YVQPQLRRPF ELLIAAAMER NPTQFQLPNE LTCTTALPGS SKRRRKEETT GKNVKRTQHE LDHNGLVPLP VKVCFTCNRS CRVAPLIQCD YCPLLFHMDC LEPPLTAMPL GRWMCPNHIE HVVLNQKNLT LSNRCQVFDR FQDTISQHVV KVDFLNRIHK KHPPNRRVLQ SVKRRSLKVP DAIKSQYQFP PPLIAPAAIR DGELICSGVP EESQTHLLNS EHLATQAEQQ EWLCSVVALQ CSILKHLSAK QMPSPWDSEQ TEKADIKPVI VTDSSITTSL QTADKAPLPS HYPLSCPSAV STQNSLGCSP PHQPPTLEDI SCSSCVEKSK KAPCGTANGP VNTEIKANGP HLYSSPTDST DPRRLPGANT PLPGLTHRQG WPRPLTPPSA GGLQNHVGII VKTENATGPS SCPQRSLVPV PSLPPSIPSS CASIENTSTL HRKTVQSQIG PSSTESRPLG SPPNATRVLT PPQAAGDSIL ATGANQRFCS PAPSSDGKVS PGTLSIGSAL TVPSFPANST AMVDLTNSLR AFMDVNGEIE INMLDEKLIK FLALQRVHQL FPSRVQASPG NVGTHPLASG GHHPEVQRKE VQARAVFCPL LGLGGAVNMC YRTLYIGTGA DMDVCLTNYG HCNYVSGKHA CIFYDENTKH YELLNYSEHG TTVDNVLYSC DFSEKTPPTP PSSIVAKVQS VIRRRRHQKQ DEEPSEEAAM MSSQAQGPQR RPCNCKASSS SLIGGSGAGW EGTALLHHGS YIKLGCLQFV FSITEFATKQ PKGDASLLQD GVLAEKLSLK PHQGPVLRSN SVP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PHF12 (PHD Finger Protein 12 (PHF12))
- 别名
- Phf12 (PHF12 产品)
- 别名
- PF1 Protein, 2410142K10Rik Protein, F630045O13 Protein, mKIAA1523 Protein, PHD finger protein 12 Protein, PHD finger protein 12 S homeolog Protein, PHF12 Protein, phf12 Protein, phf12.S Protein, Phf12 Protein
- 背景
- PHD finger protein 12 (PHD factor 1) (Pf1),FUNCTION: Transcriptional repressor acting as key scaffolding subunit of SIN3 complexes which contributes to complex assembly by contacting each core subunit domain, stabilizes the complex and constitutes the substrate receptor by recruiting the H3 histone tail. SIN3 complexes are composed of a SIN3 scaffold subunit, one catalytic core (HDAC1 or HDAC2) and 2 chromatin targeting modules. SIN3B complex represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2. SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription. May also repress transcription in a SIN3A-independent manner through recruitment of functional TLE5 complexes to DNA (By similarity). May also play a role in ribosomal biogenesis (PubMed:27956701). {ECO:0000250|UniProtKB:Q96QT6, ECO:0000269|PubMed:27956701}.
- 分子量
- 109.5 kDa
- UniProt
- Q5SPL2
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