DROSHA Protein (AA 1-1373) (Strep Tag)
-
- 抗原 See all DROSHA 蛋白
- DROSHA (Drosha, Ribonuclease Type III (DROSHA))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1373
- 宿主
- 小鼠
-
资源
- Tobacco (Nicotiana tabacum)
- 标记
- This DROSHA protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
-
MQGNTCHRMS YHPGRGCPRG RGGHGARPSA PAFRPQNLRL LHPQQPPAQY QYEPPSAPSS SYSNSQAPSF MPPRPDFVPY PPPAAPSAQG PLPPCPVRPP YPNHQMRHPF PVPPCFPPMP PPMPCPNNPP ASGAPPGQGT FPFMVPPPSM PHPPPPPVMP QQVNYQYPPG YSHSFPPPGF NSYQNNSSSF PPSANSSSTP HFRHLPPYSL PKAQNERRSP ERLKHYDDHR HRDHSHGRGE RHRSLERRER GRSPERRRPE SRYRSDYDRG RTPPPRHRSY ERSRERDRER HRHREARRSP SLERSYKKEY KRSGRSYALP VAPEPAGCTP ELPGEMIKTT ESWAPPPENV NHRSPSREKK RARWEEEKDR WSDSQGSGKE KNYTSIKEKE AEEVPPEKTE EEEEELLKPV WIRCTHSESY YSSDPMDQVG DSTVVGTSRL RDLYDKFEEE LGNRQEKAKA ARPPWEPPKT KLDEDLESSS ESECETDDDS TCSSSSDSEV FDVIAEIKRK KAHPDRLHDE LWYNDPGQMN DGPLCKCSAK ARRTGIRHSI YPGEEAIKPC RPMTNNAGRL FHYRITVSPP TNFLTDRPTV IEYDDHEYIF EGFSMFAHAP LTNIPLCKVI RFNIDYTIHF IEEMMPENFC VKGLELFSLF LFRDILELYD WNLKGPLFED SPPCCPRFHF MPRFVRFLPD GGKEVLSMHQ ILLYLLRCSK ALVPEEEIAN MLQWEELEWQ KYAEECKGMI VTNPGTKPSS VRIDQLDREQ FNPEVITFPI IVHFGIRPAQ LSYAGDPQYQ KLWKSYVKLR HLLANSPKVK QTDKQKLAQR EEALQKIRQK NTMRREVTVE LSSQGFWKTG IRSDVCQHAM MLPVLTHHIR YHQCLMHLDK LIGYTFQDRC LLQLAMTHPS HHLNFGMNPD HARNSLSNCG IRQPKYGDRK VHHMHMRKKG INTLINIMSR LGQDDPTPSR INHNERLEFL GDAVVEFLTS VHLYYLFPSL EEGGLATYRT AIVQNQHLAM LAKKLELDRF MLYAHGPDLC RESDLRHAMA NCFEALIGAV YLEGSLEEAK QLFGRLLFND PDLREVWLNY PLHPLQLQEP NTDRQLIETS PVLQKLTEFE EAIGVIFTHV RLLARAFTLR TVGFNHLTLG HNQRMEFLGD SIMQLVATEY LFIHFPDHHE GHLTLLRSSL VNNRTQAKVA EELGMQEYAI TNDKTKRPVA LRTKTLADLL ESFIAALYID KDLEYVHTFM NVCFFPRLKE FILNQDWNDP KSQLQQCCLT LRTEGKEPDI PLYKTLQTVG PSHARTYTVA VYFKGERIGC GKGPSIQQAE MGAAMDALEK YNFPQMAHQK RFIERKYRQE LKEMRWEREH QEREPEEAED IKK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product DROSHA 蛋白
-
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- DROSHA (Drosha, Ribonuclease Type III (DROSHA))
- 别名
- Drosha (DROSHA 产品)
- 别名
- im:7150667 Protein, zgc:158612 Protein, ETOHI2 Protein, HSA242976 Protein, RANSE3L Protein, RN3 Protein, RNASE3L Protein, RNASEN Protein, 1110013A17Rik Protein, AI874853 Protein, Etohi2 Protein, Rn3 Protein, Rnasen Protein, RGD1307626 Protein, ribonuclease type III, nuclear Protein, Ribonuclease 3 Protein, ribonuclease 3 Protein, drosha ribonuclease III Protein, drosha, ribonuclease type III Protein, rnasen Protein, Thicy_0885 Protein, Sinme_0866 Protein, Isova_2087 Protein, Sphch_0770 Protein, DROSHA Protein, Drosha Protein
- 背景
- Ribonuclease 3 (EC 3.1.26.3) (Protein Drosha) (Ribonuclease III) (RNase III),FUNCTION: Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26255770). Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. {ECO:0000250|UniProtKB:Q9NRR4, ECO:0000269|PubMed:26255770}.
- 分子量
- 158.8 kDa
- UniProt
- Q5HZJ0
- 途径
- Regulatory RNA Pathways
-